Figure 1

Figure 1

A library of synthetic cannabinoids (SCs) was screened for their ability to reduce the high velocity seizure-like swim behavior of 5 day old zebrafish larvae. Compounds were screened at (A) 250 µM and (B) 10 µM. Each data point represents the mean velocity change in swim behavior of six fish treated with an individual compound. The red data points represent compounds that failed to go into solution or identified as toxic after 90-min exposure. (C) Summary of compound effects after screening 370 SCs at 250 and 10 µM. The threshold for inhibition of seizure activity was determined as a reduction in mean swim velocity of ≥40%. (D) The total number of compounds identified as positive from the 250 and 10 µM library screen. (E) Heat map representing the 20 compounds which successfully reduced the mean swim velocity by >40% in the 250 and 10 µM screening.