Figure 4-figure supplement 1

Figure 4-figure supplement 1

Compared to the average speed of DMSO WT controls, treatment with (A) wact-405, (B) wact-488, (C) wact-526, (D) PI3K inhibitor PI-103, and (E) anthralin reduced the average speed of WT siblings while the expected decrease in movement speed in treated ryr1b was not observed. The difference in average speed of ryr1b+p38 inhibitor compared to WT controls (i.e. WT siblings+DMSO vehicle) is higher than expected given the effects of genotype and chemical alone, indicative of a positive chemical-genetic interaction. Chemical-genetic interactions were not observed for the remaining 18 hits from the C. elegans screen when tested in this assay (Figure 4—figure supplement 2–3F–W). Data are presented as mean ± SEM movement speed (mm/s) normalized to DMSO-treated WT siblings for n = 2 independent experiments for (A–E), (G), (H), (K), (L), and R), where sample size n = 19 or 20 for each treatment group in a set and n = 1 experiment and sample size n = 9 or 10 for the remainder. Statistical analysis by two-way ANOVA followed by Tukey’s multiple comparisons post-test. *p<0.05, **p<0.01, ***p<0.001.