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Figure 1

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ZDB-IMAGE-200509-14

Genes

Antibodies

Publication

Furlan et al., 2020 - Calsequestrins New Calcium Store Markers of Adult Zebrafish Cerebellum and Optic Tectum

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Figures for Furlan et al., 2020

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Figure Caption

Figure 1

Casq mRNA in selected tissues from adult zebrafish (A). The levels of RNA expression are relative to two different housekeeping genes, beta-2-microglobulin (B2M) and elongation factor1-α (EF1α). Mean values and standard error were determined from triplicate runs of the qPCR assay in three different tissue preparations. (B). Anti-Casq antibodies reactivity on total homogenates (40 μg per lane) obtained from pooled (N = 3) skeletal muscle, heart, and brain of adult zebrafish. In the control lanes, (anti-rabbit) blot incubation was performed in the presence of anti-rabbit conjugate with alkaline phosphatase only. Molecular mass markers (in kDa) are indicated. (C)In vitro deglycosylation of Casqs. Immunodetection of Casq by CC antibody in membrane-enriched fraction P4 (see “Materials and Methods” section) after endoglycosidase F digestion, with 10 μg loaded per lane.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neuroanat.