ZFIN Image: Furlan et al., 2020, Figure 1

Image

Figure Caption

Figure 1

Casq mRNA in selected tissues from adult zebrafish (A). The levels of RNA expression are relative to two different housekeeping genes, beta-2-microglobulin (B2M) and elongation factor1-α (EF1α). Mean values and standard error were determined from triplicate runs of the qPCR assay in three different tissue preparations. (B). Anti-Casq antibodies reactivity on total homogenates (40 μg per lane) obtained from pooled (N = 3) skeletal muscle, heart, and brain of adult zebrafish. In the control lanes, (anti-rabbit) blot incubation was performed in the presence of anti-rabbit conjugate with alkaline phosphatase only. Molecular mass markers (in kDa) are indicated. (C)In vitro deglycosylation of Casqs. Immunodetection of Casq by CC antibody in membrane-enriched fraction P4 (see “Materials and Methods” section) after endoglycosidase F digestion, with 10 μg loaded per lane.

Figure Data

Expression / Labeling details

Acknowledgments

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