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Fig. 9 Slbp2 is required for histone production during early development. A. Western blot analysis of the four core histones in wild-type and srh embryos. Anti-α-tubulin was used as a loading control. B. The srh developmental arrest phenotype can be rescued by injecting total histone protein into one-cell stage srh embryos. P-values were determined using a Student’s t-test. ****p< 0.0001. srh embryos injected with 5 ng (C-E) or 7.5 ng (F-I) of whole histone. C and F, lateral views imaged at 5 hpf. D and G lateral views imaged at 6 hpf. E and H were imaged at 24 hpf. I was imaged at 48 hpf. Note the head formation and eye pigmentation in H and the presence of melanocytes in I. show less