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Fig. 1 Expression Pattern of arl4aa in Zebrafish Embryos and Adults (A–C) Whole-mount in situ hybridization (WISH) of arl4aa at (A) 18 and 24 hpf, (B) 36 hpf, and (C) 48 hpf. (D and E) Cross-sections of the trunk at 24 (D) and 36 hpf (E) as shown by H&E staining (left panels) and WISH (right panels), at the level shown by the black lines in (A and B). H&E, hematoxylin and eosin; hpf, hours postfertilization; NC, notochord; DA, dorsal aorta; PCV, posterior cardinal vein. (F) Expression of arl4aa in different adult zebrafish tissues detected by semi-quantitative RT-PCR. Scale bars, 250 μm (A–C) and 50 μm (D and E).