Figure 5—figure supplement 1

Figure 5—figure supplement 1

(A) Expression of additional mesendoderm marker genes (papc, myoD, tbx6 and gsc) as determined by whole mount in situ hybridization in control MO (6 ng) or sqt (2 ng) + cyc (4 ng) MO YSL-injected embryos at bud stage. Schematic representation of the embryo views is shown in the left. The proportion of embryos with a phenotype similar to the images shown is indicated in the lower right corner (papc: control, n = 52, N = 3, sqt/cyc, n = 27, N = 3; myoD: control, n = 39, N = 3, sqt/cyc, n = 19, N = 3; tbx6: control, n = 25, N = 3, sqt/cyc, n = 20, N = 3 and gsc: control, n = 44, N = 3, sqt/cyc, n = 28, N = 3). (B) Percentage of bud stage sqt (2 ng) + cyc (4 ng) MO YSL-injected embryos showing expression of mesendoderm (hgg: n = 57, N = 4; gsc: n = 28, N = 3; myoD: n = 19, N = 3; ntl: n = 28, N = 4; papc: n = 27, N = 3; tbx6: n = 20, N = 3) and endoderm (sox32: n = 23, N = 3) marker genes, as determined by whole mount in situ hybridization. (C) High-resolution images of control MO (6 ng) or sqt (2 ng) + cyc (4 ng) MO YSL-injected embryos expressing gsc::GFP-CAAX and H2B-EGFP (injection control) at 50% epiboly (dorsal domain: control, n = 10, N = 4; sqt/cyc, n = 7, N = 4; lateral domain: control, n = 9, N = 4; sqt/cyc, n = 7, N = 4) and germ ring (dorsal domain: control, n = 7, N = 4; sqt/cyc, n = 8, N = 4; lateral domain: control, n = 8, N = 4; sqt/cyc, n = 7, N = 4). The position along the dorsal-ventral axis is indicated at the top. Images correspond to the embryos shown in Figure 5C. Scale bars: 200 µm (A), 100 µm (C).

Figure 5—figure supplement 1—source data 1.