Global and local effects of genetic deletion of papp-aa.
(A) Gross morphology of wild-type (WT), papp-aa+/-, and papp-aa-/- fish at the indicated stages. Lateral views with anterior to the left. Scale bar = 0.5 mm. (B–E) Body length (B), somite number (C), and head-trunk angle (D) were measured at 24 hpf and head-trunk angle (E) at three dpf. Data shown are means ± SEM. n = 6 ~ 24 fish/group. No statistical significance was found using one-way ANOVA followed by Tukey’s multiple comparison test. (F) Fish of the indicated genotypes were raised in E3 medium to three dpf. NaR cells were imaged before and 8 hr after the low [Ca2+] treatment. The apical opening was marked by dotted lines. Scale bar = 10 µm.
Excel spreadsheet containing quantitative data for Figure 2—figure supplement 3.
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