Fig. 6

Fig. 6

a Representative flow cytometric analysis of cells in scales at 1 day post-fracture (dpf) from a trap:GFP; osterix:mCherry double-transgenic animal. Gated regions in the left panel indicate the Hoechst 33342^high (Hoe^high) cell fraction and osterix:mCherry⁺ Hoe^low fraction (“EV”). Cells in the Hoe^high fraction are displayed in the right panel to further divide into three populations, trap:GFP⁻osterix:mCherry⁺ (“mCh⁺”), trap:GFP^lowosterix:mCherry⁺ (“GFP^low”), and trap:GFP^high (“GFP^high”). b Principal component analysis (PCA) based on the read per million (RPM) of each sample. c, d Hierarchical clustering of selected OC-related c and OB-related genes d in the mCh⁺, GFP^low, GFP^high, and EV fraction in the fractured scale. e Gene ontology enrichment analysis of highly expressed genes in the mCh⁺, GFP^low, GFP^high, and EV fraction. Experiments were performed once with two biological replicates.