Figure 6

Figure 6

SOCS3 Re-expression in MUT NSCs Prevents NSC Exhaustion and Restores Self-Renewal

(A) Top: Socs3 gene. ChIA-PET interactions, SOX2 peaks, and ChIA-PET reads in WT NSCs. Bottom: loss of interactions in Sox2-MUT NSCs.

(B) Growth curves of MUT NSCs, not transduced (MUT) or transduced (MUT Socs3) with a Socs3-GFP-expressing lentivirus, and of WT controls (WT or WT Socs3).

(C) Images (phase-contrast) of MUT or Socs3-transduced MUT NSCs 3 days after passage 12; neurospheres develop only from Socs3-transduced NSCs. For comparison, WT NSCs.

(D) FACS analysis (GFP) of MUT, WT, and MUT Socs3 cells at the indicated passage number. With passaging, the fraction of GFP⁺ NSCs progressively increases in MUT Socs3 NSCs, eventually reaching 100%.