Figure 2

Figure 2

Dynamic evolution of the Ca²⁺ domains. (A,B) The volume representation depicts the active pixels from the binary stacks, clustered in domains of different colors of PTZ and MO larvae. The frame on the left is the projection of active domains during the entire period. The LFP events in red have high Ca²⁺ recruitment (>700 voxels). (C) The shaded sections in A and B are the spatial patterns of activation during the interictal-like events (frame 1) and the generalized events (frame 2) in the PTZ (red) and MO (blue). (D) Difference of the spatio-temporal morphology of PTZ and MO clusters with respect to WT clusters. The maximum surface occupied by Ca²⁺ clusters is plotted in relation to their duration. The color bars represent the p-value of the difference with the WT model. We report only the significative differences (p-value ≤ 0.05). 4 recordings for each larva, n = 3 larvae for each condition. (E) Comparison of the amounts of detected voxels for the three models in different anatomical regions. (F) Anatomical localization of the origin of the recruited neuronal clusters with volume > 300 voxels. The data have been normalized to the total number of active domains measured in each recording session (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).