Figure 5

Figure 5

Overexpressions of Sox2 rescued the motor neuron defects in Sox2 mutant embryos. (A) Confocal imaging analysis of primary motor neurons in three different groups at 48 and 72 hpf. Scale bar = 50 μm. (B) Quantification of zebrafish embryos with abnormal PMNs (n = 120, 241, and 132, respectively). (C) Quantification of the undifferentiated neuronal cells in the three different groups (n = 4). Values with ** and *** above the bars are significantly different (P < 0.01 and P < 0.001, respectively). (D) Quantification of the length of CaP axons in the four different groups (n = 12 and 6). Circle means control, triangle means Sox2 mutant, square means Sox2 mutant +mRNA, and inverted triangle means Sox2 mutant +construct. Values with different letters on the top of bars are significantly different (P < 0.05). (E) Multisite Gateway cloning (three-way multisite reaction) to generate a motor neuron specific Tol2 construct for expression of Sox2 mRNA.