Figure 2

Figure 2

Effect of 5-fluorouracil (5FU) and FOLFIRI ((leucovorin + 5-fluorouracil (5FU) + irinotecan) treatments on HT29-cells cultured in a 3D model. (A) Comparison between percentages of viable cells (by absorbance fold-change detection) after administration of 5FU at 1-10-100 μM in 2D cultures and in both 3DN and 3DT models. (B) Calculation of 5FU and FOLFIRI 3D IC₅₀ by nonlinear regression. (C) MIB1 immunohistochemistry before and after administration of 3D-calculated IC₅₀ in both 3DN and 3DT; comparison of percentages of MIB1⁺ cells before and after treatment (scale bar = 100 µm). (D) EdU staining as a marker of proliferation and TUNEL staining as a marker of apoptosis, before and after 5FU treatment in 3DN and relative quantification (scale bar = 100 µm). (E) EdU and TUNEL before and after 5FU treatment in 3DT and relative quantification (scale bar = 100 µm) (* p-value < 0.05; ** p-value < 0.01; *** p-value < 0.001).