ZFIN Image: Scaletti et al., 2020, Figure 3.

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Figure Caption

Figure 3.

Kinetic characterization of MTH1-catalyzed hydrolysis of dATP, N6-methyl-dATP, N6-methyl-ATP, dGTP, and O6-methyl-dGTP.A, substrate saturation curves of MTH1 (1 nm) were produced using MTH1 reaction buffer (pH 7.5). Initial rates were determined at dATP concentrations varied between 0 and 200 μm and between 0 and 300 μm for N6-methyl-dATP and N6-methyl-ATP, respectively, and for dGTP and O6-methyl-dGTP (B) using 0–300 and 0–200 μm, respectively. Formed PP_i was detected using PPiLight^TM Inorganic Pyrophosphate Assay (Lonza) and the assay signal was converted to concentration of PP_i by including a PP_i standard curve on the assay plate.

Acknowledgments

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