ZFIN Image: Scaletti et al., 2020, Figure 2.

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Figure Caption

Figure 2.

MTH1 activity with N6-methyl-dATP and N6-methyl-ATP compared with dATP. Activity of 1 and 5 nm MTH1 was tested with 50 μm N6-methyl-dATP, dATP, ATP, and N6-methyl-ATP in MTH1 reaction buffer (pH 8.0) at 22 °C. Reaction time was 30 min and 0.2 units/ml of PPase was used to generate P_i from produced PP_i. P_i was detected by addition of malachite green reagent followed by measurement of the absorbance at 630 nm. Controls with PPase only was included and background signal was subtracted from the assay data. A P_i standard curve was included on the plate enabling determination of the concentration of formed PP_i. Graph shows mean ± S.D. from one experiment performed in quadruplicate.

Acknowledgments

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