Fig. 3

Fig. 3 Endogenous Yap Signaling Is Sufficient to Induce HSPC Production (A) Maximum intensity projection (max proj.) of confocal z stack of double transgenic embryo at 36 hpf. Kdrl+ endothelium in magenta and Ctgfa+ cells in cyan. The dashed box denotes VDA and dotted line highlights region magnified in (B) (scale bars: 500 μm in overview and 50 μm in inset). DA, dorsal aorta; PCV, posterior cardinal vein. (B) Merge and single-color slices from (B) showing the Kdrl+ membrane surrounding Ctgfa+ cells (yellow arrowheads) in the VDA. Scale bar, 20 μm. (C) Max proj. of confocal z stack of 36 hpf WT embryo. Double fluorescent in situ hybridization of endogenous mRNA expression for fli1a (magenta) and ctgfa (cyan). Bright stripe of ctgfa in notochord (yellow arrowhead) mirrors GFP expression in the reporter line. The dashed box denotes the VDA region shown in Figure 3D. Scale bar, 50 μm. (D) Merge and single-color slices from confocal z stack showing a pair of neighboring fli1a+ ctgfa+ cells in the VDA. Scale bar, 10 μm. (E) qPCR in lats1/2 morphants at 36 hpf (∗∗p < 0.01. Error bars indicate SEM). (F) In vivo imaging for CD41:GFP in the CHT at 72 hpf in control and lats1/2 morphants. Scale bar, 200 μm. (G) Quantification of fluorescent images in (F) (n = 9; unpaired Student’s t test, ∗∗p < 0.01. Error bars indicate SD).