Figure 8

Figure 8

Effect of WNK1 pathway inhibitors on HCC in transgenic fish overexpressing endothelial cell-specific wnk1a. (A–F) Representative images of H&E-stained liver sections from HBx, src(p53-) fish and two lines (TG1 and TG2) of HBx, src(p53-) transgenic fish overexpressing wnk1a in endothelia. Scale bar = 20 μm. (G–L) Representative images of PCNA staining of liver sections from fish as above. (M) Distribution of histopathological diagnosis of H&E-sections as shown in panels (A–F). The statistical significance was analyzed by Chi-square test. ****: p ≤0.0001. (N) Average immunoreactive score (IRS) of proliferating cell nuclear antigen (PCNA) staining as shown in panels (G–L). The statistical significance was analyzed by unpaired Student’s t-test. *: 0.01< p ≤0.05; **: 0.001< p ≤0.01.