Figure 3

Figure 3

Effect of VEGF receptor inhibitor (PTK787), WNK inhibitor (WNK463) and OSR1/SPAK inhibitor (Closantel) on proliferation of xenotransplanted hepatoma cells. (A) Representative images of embryos before and after treated with DMSO (vehicle), PTK787, WNK463 or Closantel. Fish were immersed with drugs for 2 days beginning at 1 dpi (day postinjection) of hepatoma cells. (B,C) Distribution of embryos with decreases (green zone), unchanged (blue) and increases in tumor-associated fluorescence area (B) and intensity (C) after drugs. Total number of embryos was 24. The statistical significance was analyzed by Chi-square test. ns: non-significant, p > 0.05 comparison between different inhibitors. #: 0.01 < p ≤ 0.05; ####: p ≤ 0.0001 compared to the 0.1%DMSO control. **: 0.001 < p ≤ 0.01 compared PTK787 and WNK463. (D,E) Percentage changes of average fluorescent area (D) and average fluorescent intensity (E) at 3 dpi relative to 1 dpi. The statistical significance was analyzed by unpaired Student’s t-test. ns: non-significant, p > 0.05; *: 0.01 < p ≤ 0.05; ***: 0.0001 < p ≤ 0.001 compared to the 0.1% DMSO control.