Fig 1

Fig 1

(A) Abundances of wt Vibrio, Δmot, and Δche during monoassociation. Plotted are medians and interquartile ranges (n ≥ 17 animals/marker). Significant differences between each mutant and wt Vibrio determined by Mann-Whitney (purple asterisks: Δmot; cyan asterisks: Δche). ***p < 0.0001, **p = 0.0002. Inset shows median bacterial abundances in the water environment from each replicate experiment across all time points. (B) Experimental timeline of Aeromonas–Vibrio competition. (C) Intestinal abundances of Aeromonas and wt or mutant Vibrio strains during different competition schemes. Aeromonas abundances while alone during monoassociation are shown for statistical comparison. Letters denote significant differences between Aeromonas treatments. Lines show paired Aeromonas and Vibrio abundances within individual fish. p < 0.05, Kruskal-Wallis and Dunn’s multiple comparisons test. Adjacent bars denote medians and interquartile ranges. Significant differences based on Wilcoxon between Aeromonas and each Vibrio strain are noted below each competition. (D) Abundances of wt and mutant Vibrios during competition with Aeromonas (from panel C) normalized to abundances during monoassociation at 24 hpi (from panel A). Bars denote medians and interquartile ranges. Significant differences determined by Mann-Whitney. Fold-decreases based on medians. Underlying data plotted in panels A, C, and D are provided in S1 Data. Aer, Aeromonas; hpi, hours post inoculation; wt, wild type.