|
Figure 5
FH inhibited tumor growth in TNBC xenograft zebrafish model. 200 TGFβ1 48 h treated TNBC cells labeled with a red fluorescent dye (CM‐DiI) were injected into 2dpf normal zebrafish yolk sac. After xenograft tumor model was established, the tumor models were treated with FH‐L, FH‐M, FH‐H, CDDP, and control. The fluorescence images were analyzed by a laser confocal scanning microscope. The representative pictures were observed with the laser confocal scanning microscope (original magnification, × 80). The fluorescence IOD was used to evaluate the tumor growth inhibition ratio of Fangjihuangqi Decoction on TNBC xenograft tumor in zebrafish. Image Pro Plus was used to analysis the relative IOD value. Normal represents normal wild‐type AB strain zebrafish, Mod represents the TNBC xenograft tumor model in zebrafish, CDDP represents cisplatin (15 μg/mL), FH‐L represents Fangjihuangqi Decoction (55.5 μg/mL), FH‐M represents Fangjihuangqi Decoction (111 μg/mL), FH‐H represents Fangjihuangqi Decoction (222 μg/mL). **