Social preference and anxiety-like behaviours in zebrafish. (a) Time spent in zones of the social preference test in zebrafish adults after acclimation, with Zone 1 being closest to the side tank where the stimulus shoal was placed. (b) Distance between adult focal fish and stimulus shoal in the social preference test, during the acclimation period (0–9 minutes) and test period (10–24 minutes) of the social preference test in adults. (c–e) Shoaling parameters during the shoaling test in adults. (f) Distance to bottom over time during the novel tank diving test in adults. (g) Social preference index (SPI) of the social preference test in larvae, during the acclimation (AC) phase and the social cue (SC) phase. All values are means ± standard error of the mean.
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