ZFIN Image: Yang et al., 2020, Figure 4

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Figure 4

The expression of NOTCH2 was lower in the right ventricular outflow tract of congenital heart disease (CHD) patients. A‐F, Relative mRNA expressions of NOTCH2, COL6A2, FOXP1, NRAS, SNIP1 and VEGFA were analysed using real time RT‐PCR and normalized to β‐actin. NOTCH2 exhibited lower mRNA expression in the CHD patients (n = 13) than in the controls (n = 7). G, Immunohistochemical analysis of NOTCH2 in the RVOT of the controls (N1, N2) and CHD patients (CHD‐1, CHD‐2). The heart tissues in the CHD patients showed weak cytoplasmic staining for NOTCH2 (bar = 100 µm). H, NOTCH2 protein was assessed in the CHD patients (n = 9) and the controls (n = 4) with WB. NOTCH2 protein expression was lower in the CHD patients than in the controls. The data are presented as the mean ± SEM. Statistical significance is shown as *P < .05 vs controls, **P < .01 vs controls, and ***P < .001 vs controls

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