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Fig. S2

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Publication: Ma et al., 2020 - Structural Neural Connectivity Analysis in Zebrafish With Restricted Anterograde Transneuronal Viral Labeling and Quantitative Brain Mapping
All Figures: Figures for Ma et al., 2020

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Fig. S2

Retina injection and tissue clearing. (A)Examples of RFP expression in the retina of unpigmented (PTU-treated) animals 3 days after virus injection into the temporal retina. Images are lateral views, with rostral side to the left and dorsal side to the top. Yellow dashed lines outline the eye (outer oval) and lens (inner oval). (B,C) Tissue clearing with sRIMS solution. Whole-mount ERK1/2 immunolabeling without (B) or with sRIMS clearing (C). Orthogonal views (XY, XZ, and YZ) of confocal image stacks are shown, centered just bellowed the cerebellum (intersect of yellow lines). Ventral structures are not visible without sRIMS clearing. Scale bars are 100 μm.

Acknowledgments

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