Fig. 2-S2
Feeding a high fat meal did not impair subsequent fatty acid intake.
( A?C) Fatty acid was labeled with green fluorescence in BODIPY-C5 ( Carten et al., 2011). BODIPY-C5 (in BSA complex) was delivered to zebrafish larvae that had been fed high fat (HF) meal for 6 hr, the same as the EEC activity assay. Within 5 min of delivery, green BODIPY-C5 was distributed throughout the entire zebrafish intestinal lumen. ( D?F) Fatty acids were labeled with red fluorescence in BODIPY-C12 ( Carten et al., 2011). BODIPY-C12 was delivered to zebrafish larvae that had been fed HF meal for 6 hr, the same as the EEC activity assay. Within 5 min of delivery, red BODIPY-C12 was distributed throughout the zebrafish intestinal lumen.