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Fig. 7-S1
The effect of palmitate feeding on EEC morphology and function.
( A) Confocal image of Tg(neurod1:RFP) zebrafish intestine in control and 6 hr post palmitate feeding zebrafish larvae at 6dpf. ( B) EEC morphology score in control and palmitate fed zebrafish larvae. ( D) EEC response to palmitate following different dietary manipulations. 6 dpf Tg(neurod1:Gcamp6f) zebrafish were untreated (n = 6) or fed for 6 hr with BSA (n = 3), ZM000 (larvae zebrafish food) (n = 4), HF meal (6.25% chicken egg yolk) (n = 11), or palmitate (n = 4). Only egg yolk and palmitate feeding reduced the EECsresponse to palmitate stimulation. ( E) EEC response to glucose stimulation following 6 hr of palmitate feeding. ( F?G) Representative confocal image of control (n = 5) and 6 hr palmitate treated (n = 7) intestine in Tg(neurod1:TagRPF); Tg(ef1?:xbp1?-GFP) zebrafish. ( H) Quantitative real-time PCR measurement of relative bip and grp94 mRNA levels from dissected tracts in control (n = 4 biological replicate pools of 20 fish, three technical replicates) and 6 hr palmitate treated (n = 3 biological replicate pools of 20 fish, three technical replicates). Student t-test was used in C, E and H, one-way ANOVA with post-hoc Tukey test was used in D for statisc analysis. *p<0.05, ns p>0.05, not signficicantly different.