Fig. 4-S3

( A) Confocal projection of 6 dpf TgBAC(neurod1:EGFP); Tg(ubb:secA5-TdTomato) intestine. Apoptotic cells were labeled by secA5-TdTomato as red (yellow arrow). ( B) Schematic view of labeling apoptotic cells using secA5-TdTomato (secreted Annexin5-TdTomato). During apoptosis, phosphatidylserine flips to the outer cellular membrane. The secA5-TdTomato is then able to bind to phosphatidylserine and label the apoptotic cells red. ( C) Confocal image of TgBAC(neurod1:EGFP); Tg(ubb:secA5-TdTomato) zebrafish intestine following 6 hr of the high fat (HF) meal. In all the samples that were examined (n = 10), no apoptotic EECs were observed. ( D) Quantification of average EEC cell volume in zebrafish from control, and 4 hours-10 hours post HF fed zebrafish. Each dot represents an individual animal with 30?60 EECs assessed per animal. One-way ANOVA with post-hoc Tukey test was used in F for statistical analysis.