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Fig. 3 Identification of Functionally-Distinct Rhabdomyosarcoma Cell Types Using Photo-Convertible Dendra2-H2b and Single-Cell Fate Mapping (A?E) A prkdc?/?, il2rga?/? zebrafish engrafted with H2b-Dendra2+ RD cells. Cells were engrafted into the peri-ocular muscle and imaged at 7 days post transplantation. Epifluorescent image of head region (A) and higher magnification confocal images (B, 100×; C?E, 200× magnification). Images were taken before 405 nm UV photoconversion (B and C) or immediately post UV exposure (D and E; merged image, E, shows excitation using both 488 and 563 nm laser). (F) Serial imaging of three photo-converted RD ERMS cells. Cells have been pseudo-colored and tracked at 0, 24, 48, 96, and 168 h after photoconversion. Cell fates are pictorially depicted within lower panels with migration denoted by wavy lines. (G) Quantification of single-cell behaviors in RD ERMS and Rh41 ARMS cells. Number of cells imaged with each phenotype are noted within bar graphs. Scale bar represents 0.1 cm (A); 50 ?m (B?E); 200 ?m (F). See also Figures S2 and S3.
Reprinted from Cell, 177(7), Yan, C., Brunson, D.C., Tang, Q., Do, D., Iftimia, N.A., Moore, J.C., Hayes, M.N., Welker, A.M., Garcia, E.G., Dubash, T.D., Hong, X., Drapkin, B.J., Myers, D.T., Phat, S., Volorio, A., Marvin, D.L., Ligorio, M., Dershowitz, L., McCarthy, K.M., Karabacak, M.N., Fletcher, J.A., Sgroi, D.C., Iafrate, J.A., Maheswaran, S., Dyson, N.J., Haber, D.A., Rawls, J.F., Langenau, D.M., Visualizing Engrafted Human Cancer and Therapy Responses in Immunodeficient Zebrafish, 1903-1914.e14, Copyright (2019) with permission from Elsevier. Full text @ Cell