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Fig. S2 Engraftment of H2b-EGFP+ Rhabdomyosarcoma Cells into the Peri-Ocular Muscle of prkdc−/−, il2rga−/− Zebrafish, Related to Figure 3 (A–H) Live-cell imaging and quantitation of RMS tumor cells over time (RD ERMS: A–D; Rh41 ARMS: E–H). Merged brightfield and fluorescent image of the head region of a prkdc−/−, il2rga−/− zebrafish engrafted with EGFP+ tumor cells for 21 days post-transplantation (dpt; A and E). Serial Z-stack confocal image at 0 dpt (B and F) and 21 dpt (C and G). Quantification of EGFP+ cells over time (D and H). (I–Q) Histological analysis of tumors engrafted into the peri-ocular muscle and analyzed at 21 days post-treatment (RD ERMS: I–M; Rh41 ARMS: N–Q). Hematoxylin and eosin stained sections (I and N, low magnification; and J and O, high magnification of boxed region). Immunohistochemistry for Ki67 (K and P) and TUNEL (M and Q) with average percent positive cells ± standard deviation noted (n ≥ 3 fish/tumor). Scale bar represents 0.1 cm (A and E); 50 μm (B, C, F, and G), 200 μm (I and N); 50 μm (J–M and O–Q).
Reprinted from Cell, 177(7), Yan, C., Brunson, D.C., Tang, Q., Do, D., Iftimia, N.A., Moore, J.C., Hayes, M.N., Welker, A.M., Garcia, E.G., Dubash, T.D., Hong, X., Drapkin, B.J., Myers, D.T., Phat, S., Volorio, A., Marvin, D.L., Ligorio, M., Dershowitz, L., McCarthy, K.M., Karabacak, M.N., Fletcher, J.A., Sgroi, D.C., Iafrate, J.A., Maheswaran, S., Dyson, N.J., Haber, D.A., Rawls, J.F., Langenau, D.M., Visualizing Engrafted Human Cancer and Therapy Responses in Immunodeficient Zebrafish, 1903-1914.e14, Copyright (2019) with permission from Elsevier. Full text @ Cell