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Figure 2

ID
ZDB-IMAGE-200325-67
Source
Figures for Timonina et al., 2020
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Figure Caption

Figure 2

Comparison of the panx1a Y205F and Y205A mutants. (A) Neuro 2a cells transfected with WT panx1a-EYFP (left) and the Y205F-EYFP mutant (right) both showed membrane localization. Scale bar: 10µm. (B) Western blot analysis revealed similar glycosylation patterns when the Y205F mutant was compared to WT panx1a. (C) Cell surface biotinylation assay showing that Y205F was present at the membrane of transfected Neuro 2a cells. Cell lysates after NP40 buffer lysis (Input) showed expression of both intracellular (ß-actin; Y205A) and membrane proteins (panx1a; Y205F). The streptavidin pull-down fractions (Biotinylation) showed that all intracellular proteins were depleted, with panx1a and Y205F found in the protein fraction from the cell surface. ß-actin served as an internal control, showing no bands in the biotinylation fraction. Expressed proteins were detected using an anti-GFP antibody and an anti-ß-actin antibody as the control.

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