ZFIN Image: Timonina et al., 2020, Figure 1

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Figure Caption

Figure 1

Expression and localization of panx1a WT and mutants in Neuro 2a cells. (A) Sequence alignment of panx1a with pannexin orthologs and paralogs and the Caenorhabditis elegans INX-6 protein. (B) Topological representation of a panx1a subunit showing the positions of both aromatic residues at the transmembrane and intracellular loop borders. The image was generated using Protter [27]. (C) Localization of EYFP-tagged wild type panx1a and mutants in transfected Neuro 2a cells. Scale bar: 10µm. (D) Western blot analysis of transfected EYFP-tagged panx1a and mutants. The three bands correspond to nonglycosylated panx1a (Gly0), the high mannose glycosylation (Gly1), and the complex glycosylation (Gly2) forms. Expressed proteins were detected using an anti-GFP antibody and an anti-ß-actin antibody as the control.

Acknowledgments

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