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Figure Caption
Fig. 9-S1
Pronephros development occurs normally in spzl mutants.
( A–B) Confocal images of cross sections from 48 hpf WT and spzl mutant embryos stained for NaK ATPase (green). Phalloidin (magenta) labels the apical membrane of the pronephros. Scale bar = 20 µm ( C) Whole mount immunofluorescence stain of NaK ATPase labeling thepronephros in WT (top) and spzl-/- (bottom). Arrows point to pronephros. Scale bar = 500 µm.
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