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Figure Caption
Fig. 7 Effects of diethylstilbestrol (DES) on female spawning: ovarian morphology. Panels A and B: Left and right sides of female C62 before DES treatment at 25.5 WPF (O with black arrows indicate large follicles, black outlines with white arrows denote vents). Panels C and D: Left and right sides of female C62 after 28 days of 1 μg/L DES exposure, 29.5 WPF, note the atretic ovaries and increased vent size (black outline and white arrow). All DES females in the study exhibited regression of the ovary. Images were taken at 8× magnification. Scale bar represents 1 mm.
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Reprinted from Mechanisms of Development, 160, Brantley, N., Lessman, C.A., In vivo assessment of gonad status, secondary sex characteristics and spawning in transparent Casper zebrafish, 103582, Copyright (2019) with permission from Elsevier. Full text @ Mech. Dev.