ZFIN Image: Brantley et al., 2019, Fig. 5

Image

Figure Caption

Fig. 5 Effects of diethylstilbestrol (DES) on testes and vent of Casper male zebrafish. Panels A and B: Image of left and right testes, respectively, and vent of a sexually mature male (17 WPF) before DES treatment. Black arrowheads point to opaque lower area of testes connecting to vent (white arrows). Panels C and D: Image of left and right testes, respectively, and vent of sexually mature male (21 WPF) after 4 weeks of 0.1 μg/L DES treatment. Testes are notably more transparent without obvious connection to vent. Images show reduction in testes opacity and increase in vent size after treatment. Images were taken at 12× magnification. Scale bar represents 1 mm.

Acknowledgments

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Reprinted from Mechanisms of Development, 160, Brantley, N., Lessman, C.A., In vivo assessment of gonad status, secondary sex characteristics and spawning in transparent Casper zebrafish, 103582, Copyright (2019) with permission from Elsevier. Full text @ Mech. Dev.