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Figure 2

ID: ZDB-IMAGE-200314-57
Publication: Rojas-Benítez et al., 2020 - Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
All Figures: Figures for Rojas-Benítez et al., 2020

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Figure 2

Aberrant somite shape and horizontal myoseptum in Elp3 morphants. To show somite boundary we detected β-dystroglycan in (A) control and morphant 48 hpf embryos, nuclei were stained with TO-PRO. Images representative of three independent experiments. Horizontal myoseptum is indicated with a white arrowhead. Scale Bar 50 µm. Also, somite angle (B) and area (C) were measured (N = 3, n = 50, t-Student. *** p < 0.005). Myomesin was also detected (D) in 48 hpf control and morphant embryos (N = 3, n = 50, t-Student). Scale bar 10 µm. (E) To evaluate muscle function coiling was measured counting twitches per minute in 30 hpf embryos (N = 3, n = 50, t-Student; ns non-significant).

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