IMAGE

Fig. 2

ID: ZDB-IMAGE-200306-52
Publication: Lee et al., 2020 - REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects
All Figures: Figures for Lee et al., 2020

Image

Figure Caption

Fig. 2 REEP5 expression shows consistent SR staining pattern in cardiac myocytes.

a Immunofluorescence analysis of endogenous REEP5 expression (green) and phalloidin-rhodamine staining (red) in CMNCs. Right panel, line-scan analysis (from yellow line) demonstrates SR expression pattern in CMNCs. Scale, 10 μm. b Immunofluorescence of isolated adult mouse cardiac myocytes with REEP5 (blue) co-stained with SERCA2 (green) and phalloidin-rhodamine (red). Scale, 20 μm (left panel), 10 μm (right panel). c Orthogonal projection, three-dimensional reconstructive and line-scan analyses demonstrates co-localization between REEP5, SERCA2, and phalloidin signal. (Left panel): the top panels represent cell imaging in the x–z plane, while side panels represent cell imaging in the y–z plane. All images shown are representative of approximately 40–50 total images captured per condition, n = 3 independent biological replicates.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.