Figure 2

Figure 2

Quantitation of bacterial dissemination in response to a dose titration of intravenous Mycobacterium marinum (Mm) infection. Measures of dissemination in zebrafish larvae four days after intravenous infection with 25, 100 or 400 cfu Mm ± 400 µM isoniazid (I) (n = 24, 28, 22 and 25, respectively). (a) Representative images are shown for each inoculum dose. Scale bars, 500 µm. (b) The relationship between cumulative percentage fluorescence and the number of bacterial foci responsible for this signal. (c) Fluor₅₀, calculated by interpolation of the data shown in (b). The three spatial dissemination parameters, (d) grid analysis, (e) polygon area and (f) IFD_max. Lines (b) and data points (c–f) represent individual zebrafish larvae. Lines and error bars (c–f) represent median ± IQR. p values were derived from Kruskal-Wallis tests with Benjamini, Krieger and Yekutieli correction for multiple comparisons. Data are representative of three independent experiments.