Figure 4

Figure 4

Injury activates a differential shift in PGE₂ receptor expression in the heart. (A) qPCR determination of PGE₂ receptor expression showed that in uninjured hearts, ptger3 levels were significantly higher than ptger2a or ptger4a. Gene expression was calculated relative to ptger2a. (mean ± s.e.m. n = 4–5 biological replicates; 3–5 pooled ventricles per replicate. One-way ANOVA followed by Tukey’s multiple comparisons test. ***P < 0.001). (B) Relative to uninjured hearts, expression of ptger2a was significantly upregulated, while ptger3 was significantly downregulated at 3 dpa. (mean ± s.e.m. n = 5 biological replicates; 3–5 pooled ventricles per replicate. Student’s t-test. *P < 0.05). (C) Representative in situ hybridization images of ptger2a expression in uninjured, 1, 2 and 3 dpa hearts. (n = 4 biological replicates, brackets mark approximate amputation zone; arrowheads demarcate signal within the injury zone).