Figure 6—figure supplement 3.

Figure 6—figure supplement 3.

(A) Embryos injected with a reporter construct built from a 701 bp element centered on SNP1, harboring the risk or non-risk allele as indicated. The large majority of embryos with SNP1 constructs, of either allele, were not blue, and no two blue embryos showed the same pattern. R-random integration, see below. No further copy number analysis was carried out. (B) Embryos injected with a reporter construct built from a 700 bp element centered on SNP2, harboring the risk or non-risk allele as indicated. Using the genomic DNA isolated from each embryo, PCR was carried out to determine if the reporter construct was present at all, and whether it was (S - single) present at the safe harbor locus in a single copy, (T - tandem), present at the safe harbor locus in more than one copy, or (R-random) was detectable but absent from the safe harbor locus, suggesting it integrated randomly into the genome (Kvon et al., 2020). One embryo (number 1, boxed) injected with a SNP2 construct (risk-allele) showed reporter activity in the periderm, as predicted. Quantitative PCR indicated this embryo had 8–10 copies of the reporter construct while the other T embryos had 2 copies.