ZFIN Image: Liu et al., 2020, Figure 4

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Figure Caption

Figure 4—figure supplement 3. Reporter assay for human and zebrafish <italic>PPL</italic> elements predicted by zebrafish classifier.

Transient transgenic reporter assays for enhancer candidates near human PPL and zebrafish ppl. (A) Genome browser (hg19) view for PPL -8.3kb. (B) Lateral view, anterior to the right, of an embryo at 48 hpf injected at the one cell stage with the PPL-8.3kb:gfp reporter construct. (C) Bar chart showing number of embryos with 10 or more GFP-positive periderm cells injected with the indicated construct. PPL-8.3kb:DKLF: in the enhancer element, the motif matching the KLF4 binding site has been deleted. (D) Zebrafish genome (danRer 7) browser view for ppl-10kb (E) Lateral view, anterior to the right, of an embryo at 48 hpf injected at the one cell stage with the ppl-10kb:gfp reporter construct. (F) Bar chart showing number of embryos with 10 or more GFP-positive periderm cells injected with the indicated construct. ppl-810kb:DKLF: in the enhancer element, the motif matching the KLF4 binding site has been deleted.

Acknowledgments

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