Figure 3

Figure 3

Effect of fisetin on cardiomyocytes proliferation and apoptosis. Flow cytometry experiments were carried out to measure the amount of proliferating and apoptotic cells in each experimental group. (A) Proliferation of H9c2 cells assessed by Ki67 staining. Ki67+ cells are proliferating. Results are expressed as the mean of three independent experiments performed in triplicate. One-way ANOVA. Post-hoc analysis by Tukey. No significant difference: P > 0.05. (B) Dot plot representation of H9c2 apoptotic ratio measured by annexin V/PI staining. Annexin V−/PI− cells were considered as viable, annexin V+/PI− cells were considered as early apoptotic and annexin V+/PI+ cells were considered as late apoptotic. Normoxia: control group, cells cultured under normoxia. HS/R: cells subjected to HS/R, treated with DMSO as vehicle control. HS/R + F: cells subjected to HS/R, treated with 15 μM fisetin. Results are expressed as the mean of three independent experiments performed in triplicate. Two-way ANOVA. Post-hoc analysis by Tukey. ***P ≤ 0.001.