Colocalization of celsr1a and neurod1 positive sensory enteroendocrine cells during development.
(A–C) Co-expression of celsr1a (celsr1aGFP/+) and neurod1 (Tg(neurod1:TagRFP)) in four dpf intestine; asterisk highights celsr1a+ without neurod1 expression; all pictures luminal side placed on top. (C) Overlay, shows predominant co-localization early in this cell population. Expression was seen in all larvae analyzed (n = 6). (D–E) Expression of Tg(celsr1GFP) and neurod1 in 5 month old adult zebrafish intestine. Area pictured is from the middle intestinal region. (F) Quantitation of expression and overlap between markers (n = 6, 1–4 pictures/fish/region). (G) Close up of overlay showing distinct marked cell populations.
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