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Figure EV1

ID: ZDB-IMAGE-200212-37
Publication: Ki et al., 2019 - CEP41-mediated ciliary tubulin glutamylation drives angiogenesis through AURKA-dependent deciliation
All Figures: Figures for Ki et al., 2019

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Figure Caption

Figure EV1 <styled-content toggle='no' style='fixed-case'>CEP</styled-content>41 is dispensable for generation of the centrosome and the cilium

A–D

Control or CEP41 siRNA‐transfected HUVECs were immunostained with ARL13b or γ‐Tub‐specific antibodies in (A, C). The rectangles indicate the representative cells from each immunostaining experiment presented as magnified images in the right panels. Scale bars, 20 μm. Quantification of the ciliated cell numbers in the images in (B, D) from the results of three independent experiments with ≥ 200 cells per condition (mean ± SD). ns: non‐significant (one‐way ANOVA with Tukey's post hoc test).

E, F

Wild‐type and cep41‐deficient Tg(kdrl:eGFP) zebrafish embryos were fixed for immunostaining with ARL13b‐specific antibodies at 28 hpf in (E). The rectangles indicate the arterial cilia (AC) and venous cilia (VC) in zebrafish endothelial cells. Magnified representative images are displayed in the right panels. CA, caudal artery; CV, caudal vein. Scale bars, 40 μm. Quantification of the labeled cilia observed in equivalent fields of view is presented graphically in (F). Data are shown as mean ± SD of three independent experiments (n ≥ 20 embryos per condition). Statistical significance was determined using the one‐way ANOVA followed by Tukey's post hoc test (ns: non‐significant).

Acknowledgments

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