Figure 4

Figure 4

Annotation of TRAS-labeled neurons and efferent axons in the Z-Brain standard brain reference. (A) Overview of all annotated TRAS-labeled retinorecipient cells within the wild-type and dscaml1-/- cohorts. (B) Spatial layout of TRAS-labeled neurons (dorsal view, rostral to the left) overlaid onto the Z-brain reference brain scale, for wild-type (top row) and dscaml1-/- (bottom row) cohorts. Green dots mark excitatory neurons, and magenta dots mark inhibitory neurons. (C) RGC arborization field (AFs) fluorescent intensity (a proxy for starter cell number) was similar between cohorts. (D) The total number of TRAS-labeled cells per fish was not significantly different between cohorts. (E–G) Efferent tract tracing from wild-type larvae (n = 10). Maximum Z-projection is shown for confocal image (E), traced ipsilateral tracts (F), and commissural tracts (G). Axons with similar trajectories are displayed in the same color. In (B,E–G), the horizontal dashed lines indicate the midline and the curved dashed lines indicate the optic tectum/hindbrain boundary. Scale bars are 100 μm.