Figure 2

Figure 2

Efferent projections of retinorecipient cells. (A,A′) Confocal maximal intensity projection (dorsal view) of TRAS-labeled larva, with RFP expression from VSVΔG in magenta (A) or white (A′). Axon projections can be seen in the pallium (P), optic tectum (OT), cerebellum (Cb), and hindbrain (Hb). (B–E) Maximal intensity projection confocal substacks that contained the pallium (B–B″), habenula (C–C″), hypothalamus (D–D″), and cerebellum (E–E″). RFP expression from VSVΔG (magenta) and GFP expression from the vglut2a:GFP transgene (green) are shown in B–E, while boxed region is shown at higher magnification in B′–E′ (dorsal view) and B″–E″ (lateral view), with only the RFP channel (white). Yellow arrowheads point to the same efferent projections in the dorsal and lateral views. Scale bars are 100 μm in (A,B) and 50 μm in (B′,B″). Images in the same row are shown at the same scale.