Figure 6.

Figure 6.

Sodium nitroprusside reverses the effect of glucose on neurovascular coupling. (a)–(d) Time series of neuronal activation (ΔF/F_o) and tectal vessel RBC speed in zebrafish (5 larvae/group) exposed to: (a) 20 mM mannitol for 120 h from 4 to 9 dpf. (b) 20 mM mannitol for 120 h from 4 to 9 dpf and 0.1 mM SNP for 24 h from 8 to 9 dpf. (c) 20 mM glucose for 120 h from 4 to 9 dpf. (d) 20 mM glucose for 120 h from 4 to 9 dpf and 0.1 mM SNP for 24 h from 8 to 9 dpf. (e) Frequency of peaks of neuronal activation during baseline, response and recovery time periods in mannitol or glucose exposed larvae with or without co-treatment with 0.1 mM SNP (n = 20 larvae/group). (f) RBC speed for baseline, response and recovery for same animals in (e). (g) Beating heart rate (bpm) quantified for baseline, response and recovery time periods for larvae exposed to 20 mM mannitol, 20 mM glucose with or without SNP treatment. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are mean ± s.d.