Fig. 4.

Fig. 4.

The ATF6 target gene, Bip, is highly expressed with high ATF6 reporter expression, while XBP1 expression does not correlate with caATF6. (A-C) Embryos expressing hsp70:GAL4 and 5XATF6RE:eGFP transgenes that were injected with an mCherry-tagged UAS construct to mosaically overexpress caATF6. 2-dpf embryos were heat shocked and confocal images were captured 12 h later live (A,B) or after fixation and staining (C,D), and mean intensity was quantified. (A) 5XATF6RE:d2GFP expression positively correlates with caATF6-2a-mCherry expression (R²=0.8439, P=0.0005). (B) xbp1δ-gfp expression does not significantly correlate with caATF6-2a-mCherry expression (R²=0.0077, P=0.8099). (C) 12 h post-heat shock, embryos were fixed and stained for Bip (blue) or GFP (green). Bip expression positively correlates with 5XATF6RE:d2GFP expression (R²=0.8899, P<0.0001). (D) 60× representative images showing low versus high cellular expression patterns of Bip and 5XATF6RE:d2GFP. ***P<0.001; ****P<0.0001; Pearson's correlation coefficient. Scale bars: 1 mm (A-C); 0.02 mm (D).