Motile cilia beating pattern in the central canal of 30 hpf embryos.
(A) Kymograph of motile cilia dynamics in the ventral central canal associated to Figure 2B1. Note the diversity of beating patterns of neighboring cilia. Not only cilia beat at many frequencies, but some of them also show unrepeatable patterns. Horizontal scale bar is 20 µm, and vertical scale bar is 0.5 s. (B) Corresponding power spectral density map showing the position of the different maximal frequencies for each cilium. Note that some cilia show a power spectrum with several local maxima, accounting for the asynchronicity of the beating patterns observed. In order to obtain the frequency map as in Figure 2B2, we extract only the global maximum.
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