IMAGE

Fig. 7

ID

ZDB-IMAGE-200201-11

Genes

Antibodies

Ab2-pcna

Publication

Iribarne et al., 2019 - TNFα Induces Müller Glia to Transition From Non-proliferative Gliosis to a Regenerative Response in Mutant Zebrafish Presenting Chronic Photoreceptor Degeneration

All Figures

Figures for Iribarne et al., 2019

Image

Figure Caption

Fig. 7

Proliferation of Müller glia and rod precursor cells is not activated by light-induced damage, but by TNFα in gosh mutants at 3 wpf. Wild-type and gosh mutant retinas at 3 wpf are labeled with anti-PCNA antibody. Nuclei are stained with DAPI. The transgene Tg(gnat2:GFP) visualizes cone photoreceptors. In wild-type, undamaged controls, PCNA is expressed in the CMZ, and PCNA-positive cells are observed in the INL and ONL (A). However, the PCNA signal is almost absent in gosh mutant retinas (B). Treatment for 18 h under intense light damages photoreceptors (C,D). Wild-type sibling retinas showed an increase in the number of PCNA-positive cells located in the INL and ONL. On the other hand, the PCNA signal is still absent in gosh mutant retinas. TNFα intravitreal injections increase the number of PCNA-positive cells in wild-type sibling and gosh mutant retinas (E,F). Histogram depicting the number of PCNA-positive cells (G). Bars and lines indicate mean ± SEM, n: 6–11. Black and red bars, wild-type siblings and gosh mutants. Filled bars, no treatment; Open bars, photodamaged samples; Dotted bars, TNFα-injected sample (ns, p > 0.05; ^∗p < 0.05; ^∗∗p < 0.01).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol