Figure 3

Figure 3

The in vivo toxicity against D. rerio embryos appears to be correlated with the empirical hydrophobicities of GS 1 and its photoswitchable analogues 2–20. LD₅₀ values are plotted against the HPLC (C₁₈) retention times upon elution with a standardized linear water/acetonitrile gradient: both, for the ring-closed photoforms (A, filled circles), as well as for the ring-open photoforms (B, open circles). The compound numbers are shown next to the data points. Values for the parent peptide 1 are shown as black filled circles; the original prototype 2 is color-coded in black; the data points for peptides of series i (3–5) are shown in grey, series ii (6–9) in blue, series iii (10–15) in red, series iv (16–18) in green, and series v (19, 20) in purple.