Figure 6

Figure 6

Pancreas defects are more severe when exposure to PBZ begins at early stages. Zebrafish embryos were exposed to a range of PBZ concentrations (0.34, 3.4, and 17 μM) beginning at different stages (24, 36, 48, 60, 72, and 96 hpf). Embryos were collected at 5 dpf and analyzed by whole-mount in situ hybridization with an insulin antisense riboprobe; insulin is specifically expressed in pancreas. The intestinal phenotype was categorized as (A) ‘Normal’, ‘Mild’ or ‘Severe’ defects. Images were taken from dorsal view. (B) Frequency of pancreas phenotypes was compared in zebrafish embryos treated with different concentrations of PBZ at different exposure stages. Data represent the mean of three independent experiments; 30 embryos were assessed in each treatment (N = 3. n = 90 embryos).