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Figure 4

ID
ZDB-IMAGE-200125-90
Source
Figures for Wang et al., 2019
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Figure Caption

Figure 4

Intestine defects are more severe when exposure to PBZ begins at early stages. Zebrafish embryos were exposed to a range of PBZ concentrations (0.34, 3.4, and 17 μM) beginning at different stages (24, 36, 48, 60, 72, and 96 hpf). Embryos were collected at 5 dpf and analyzed by whole-mount in situ hybridization with an ifapb antisense riboprobe; ifapb is specifically expressed in intestine. The intestinal phenotype was categorized as (A) ‘Normal’, ‘Mild’ defects or ‘Severe’ defects. Images were taken from ventral view. (B) Frequency of intestine phenotypes was compared in zebrafish embryos treated with different concentrations of PBZ at different exposure stages. Data represent the mean of three independent experiments; 30 embryos were assessed in each treatment (N = 3. n = 90 embryos).

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